Brain Mitochondrial ATP-insensitive large conductance Ca(+2)-activated K(+) channel properties are altered in a rat model of amyloid-β neurotoxicity.

Brain Mitochondrial ATP-insensitive large conductance Ca(+2)-activated K(+) channel properties are altered in a rat model of amyloid-β neurotoxicity. Exp Neurol. 2015 Mar 28; Authors: Jafari A, Noursadeghi E, Khodagholi F, Saghiri R, Sauve R, Aliaghaei A, Eliassi A Abstract Mitochondrial dysfunction is a hallmark of amyloid-beta (Aβ)-induced neuronal toxicity in Alzheimer's disease (AD). However, the underlying mechanism of how Aβ affects mitochondrial function remains uncertain. Because mitochondrial potassium channels have been involved in several mitochondrial functions including cytoprotection, apoptosis and calcium homeostasis, a study was undertaken to investigate whether the gating behavior of the mitochondrial ATP- and ChTx-insensetive-IbTx-sensitive Ca(2+)-activated potassium channel (mitoBKCa) is altered in a rat model of Aβ neurotoxicity. Aβ1-42 (4 μg/μl) was intracerebroventricularly injected in male Wistar rats (220-250). Brain Aβ accumulation was confirmed two weeks later on the basis of an immunohistochemistry staining assay, and physiological impacts measured in passive avoidance task cognitive performance experiments. Brain mitochondrial inner membranes were then extracted and membrane vesicles prepared for channel incorporation into bilayer lipid. Purity of the cell fraction was confirmed by Western blot using specific markers of mitochondria, plasma membrane, endoplasmic reticulum, and Golgi. Our res...
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research

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Are there common regulators of aging to be found among transcription factors? Sweeping, complex, tissue-specific and species-specific changes in gene expression take place over the course of aging. If these are reactions to comparatively straightforward processes of molecular damage at the root of aging, processes that are similar between species, then it is possible that there also exist at least a few regulators that are also comparatively straightforward and similar between species. Where is the leap from simplicity to complexity? Is it that the immediate reaction to damage is complicated, with a hundred different senso...
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AbstractAlzheimer ’s disease (AD) is the most common progressive neurodegenerative disorder. A defining hallmark of the AD brain is the presence of intraneuronal neurofibrillary tangles (NFTs) which are made up of abnormally modified tau, with aberrant phosphorylation being the most studied posttranslational modifi cation (PTM). Although the accumulation of tau as NFTs is an invariant feature of the AD brain, it has become evident that these insoluble aggregates are likely not the primary pathogenic form of tau, rather soluble forms of tau with abnormal PTMs are the mediators of toxicity. The most prevalent PT M on t...
Source: Molecular Neurobiology - Category: Neurology Source Type: research
Source: Nutritional Neuroscience - Category: Nutrition Authors: Source Type: research
Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
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Source: Theranostics - Category: Molecular Biology Authors: Tags: Research Paper Source Type: research
Publication date: Available online 28 August 2020Source: Journal of Sport and Health ScienceAuthor(s): Na Zhao, Jie Xia, Bo Xu
Source: Journal of Sport and Health Science - Category: Sports Medicine Source Type: research
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Source: Ageing Research Reviews - Category: Genetics & Stem Cells Authors: Tags: Ageing Res Rev Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
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Interaction of human IAPP and Aβ1-42 aggravated the AD-related pathology and impaired the cognition in mice. Exp Neurol. 2020 Sep 29;:113490 Authors: Chen HC, Cao JX, Cai YT, Du HL, Xi XX, Sun J, Yin J, Gao LP, Jing YH Abstract Alzheimer's disease (AD) and type 2 diabetes mellitus (T2DM) have a common pathology. Both diseases are characterized by local deposition of amyloid proteins in the brain or islet organ, but their phenotypes and clinical manifestation vary widely. Although the sources of islet amyloid polypeptide (IAPP) and amyloid beta (Aβ) are independent, their fibrillar sequences ...
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research
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