Antenatal glucocorticoid treatment alters sodium uptake in renal proximal tubule cells from adult offspring in a sex specific manner.

ANTENATAL GLUCOCORTICOID TREATMENT ALTERS SODIUM UPTAKE IN RENAL PROXIMAL TUBULE CELLS FROM ADULT OFFSPRING IN A SEX SPECIFIC MANNER. Am J Physiol Renal Physiol. 2015 Apr 1;:ajprenal.00047.2015 Authors: Su Y, Bi J, Pulgar VM, Figueroa JP, Chappell MC, Rose JC Abstract We have shown a sex specific effect of fetal programming on sodium excretion in adult sheep. The site of this effect in the kidney is unknown. Therefore, we tested the hypothesis that renal proximal tubule cells (RPTC) from adult male sheep exposed to betamethasone (Beta) before birth have greater sodium uptake than do RPTC from vehicle exposed males and that RPTC from females similarly exposed are not influenced by antenatal Beta. In isolated RPTC from 1-1.5 years old male and female sheep we measured sodium uptake under basal conditions and following stimulation with angiotensin II. To gain insight into mechanisms involved we also measured nitric oxide (NO) levels, angiotensin receptor mRNA levels and expression of the Na+/H+ exchanger (NHE3). Basal sodium uptake increased more in cells from Beta than in cells from vehicle exposed males (400% vs 300%, p<0.00001). Angiotensin II stimulated sodium uptake was also greater in cells from Beta exposed males. Beta exposure did not increase sodium uptake by RPTC from the females. NO production was suppressed more by angiotensin II in RPTC from Beta exposed males than in RPTC from either vehicle exposed males or females. Ou...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research