KCNJ10 (Kir4.1) is expressed in the basolateral membrane of the cortical thick ascending limb.

KCNJ10 (Kir4.1) is expressed in the basolateral membrane of the cortical thick ascending limb. Am J Physiol Renal Physiol. 2015 Apr 1;:ajprenal.00687.2014 Authors: Zhang C, Wang L, Su XT, Lin DH, Wang WH Abstract The aim of the present study is to examine the role of Kcnj10 (Kir.4.1) in contributing to the basolateral K conductance in the cortical thick ascending limb (cTAL) using Kcnj10+/+ (WT) and Kcnj10-/- (KO) mice. The patch-clamp experiments detected a 40 pS and an 80 pS K channel in the basolateral membrane of the cTAL. Moreover, the probability of finding the 40 pS K was significantly higher in the top half of the cTAL than those in the low half. Immunostaining showed that Kcnj10 staining was detected in the basolateral membrane of the cTAL but the expression was not uniformly distributed. The disruption of Kcnj10 completely eliminated the 40 pS K channel but not the 80 pS K channel, suggesting the role of Kcnj10 in forming the 40 pS K channel of the cTAL. Also, the disruption of Kcnj10 increased the probability of finding the 80 pS K channel in the cTAL, especially in the top half of the cTAL. Because the channel open probability of the 80 pS K channel in KO was similar to those of WT mice, the increase in the 80 pS K channel may be achieved by increasing K channel number. The whole cell recording further showed that K reversal potential measured with 5 mM K in the bath and 140 mM K in the pipette was the same in the WT and ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research