Authors' response: virus-host interactions in HBV-related hepatocellular carcinoma: more to be revealed?

We acknowledge Cao et al1 for being interested in our recent study on molecular features of hepatitis B related hepatocellular carcinoma (HBV-related HCC).2 As they suggested, we searched for the HBx-LINE1 chimerical transcript recently identified in a Chinese population of HBV-related HCC by Lau et al3 in our series of HCC treated in France.2 To this aim, we have selected and analysed 50 HBV-related HCC tumours which were included in our last report.2 Total RNAs were extracted using Trizol (Invitrogen) from frozen tissues and converted into cDNA by SuperScript VILO cDNA Synthesis Kit (Life Technologies). Reverse-transcribed cDNA equivalent to 40 ng of RNA were amplified by hemi-nested PCR using the primers and the protocol designed by Lau et al.3 In our laboratory, no HBX fusion transcripts were previously amplified or cloned. Each sample was screened for the detection...

Source: Gut - April 1, 2015 Category: Gastroenterology Authors: Cao, Q., Imbeaud, S., Datta, S., Zucman-Rossi, J. Tags: PostScript Source Type: research