Effects of Normoxic and Hypoxic Exercise Regimens on Monocyte-mediated Thrombin Generation in Sedentary Men

This study explores the manner in which normoxic and hypoxic exercise regimens affect procoagulant monocyte-derived microparticle (MDMP) formation and monocyte-promoted thrombin generation (TG).Forty sedentary healthy males were randomized to perform either normoxic (NET; 21%O2, n=20) or hypoxic (HET; 15%O2, n=20) exercise training (60%VO2max) for 30 min/day, 5 days/week for 5 weeks. At rest and immediately after hypoxic exercise test (HE, 100W under 12%O2 for 30 min), the MDMP characteristics and dynamic TG were measured by flow cytometry and thrombinography, respectively.The results demonstrated that acute 12%O2 exercise (i) increased the releases of coagulant factor V (FV)/FVIII-rich, phosphatidylserine (PS)-exposed, and tissue factor (TF)-expressed microparticles from monocytes, (ii) enhanced the peak height and rate of TG in monocyte-rich plasma (MRP), and (iii) elevated concentrations of norepinephrine/epinephrine, myeloperoxidase, andlnterleukin-6 in plasma. Following the 5-week intervention, HET exhibited higher enhancements of peak work-rate and cardiopulmonary fitness than NET did. Moreover, both NET and HET decreased the FV/FVIII-rich, PS-exposed, and TF-expressed MDMP counts and the peak height and rate of TG in MRP following the HE test. However, HET elicited more suppression for the HE-enhanced procoagulant MDMP formation and dynamic TG in MPR and catecholamine/peroxide/proinflammatory cytokine levels in plasma than NET. Hence,we conclude that HET is superior to...

Source: Clinical Science - March 31, 2015 Category: Biomedical Science Authors: J Wang, Y Chang, Y Chen, H Tsai, T Fu Source Type: research