Artifactual changes in the haematological values of cultured Hetroclarias hybrid blood stored at room and refrigerator temperatures

This study investigated the artifactual changes in the haematological values of Hetroclarias hybrid blood stored at room (30 °C) and refrigerator (5 °C) temperatures. Twelve apparently healthy fish weighing between 700 and 900 g were used for the study. Samples were immediately upon collection divided into two parts and haematological determinations carried out at six hourly intervals from 0 to the 36th hour and then at the 48th hour. Results showed significant reduction (p < 0.05) in PCV and RBC values at the 48th hour from the baseline value at room temperature and no significant variation (p > 0.05) at the refrigerator temperature. There were significant increases (p < 0.05) in the MCV and MCHC values from the baseline values at the 48th hour and MCH value at 36th hour at room temperature while no significant variations (p > 0.05) were observed at refrigerator temperature. There were significant reductions (p < 0.05) in the WBC count recorded at the 36th and 48th hours when compared with the baseline value at room temperature but no significant variation (p > 0.05) was observed at the refrigerator temperature. HBC values recorded did not vary significantly (p > 0.05) in both temperatures all through the study. Reliable PCV, HBC, RBC, WBC and mean corpuscular values can be obtained up to the 48th hour at refrigerator temperature while at room temperature, reliable PCV, RBC, MCV and MCHC can be obtained at the 36th hour a...

Source: Comparative Clinical Pathology - March 27, 2015 Category: Pathology Source Type: research