Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia.

Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia. Exp Neurol. 2015 Mar 19; Authors: Zheng S, Wei S, Wang X, Xu Y, Xiao Y, Liu H, Jia J, Cheng J Abstract Sphingosine kinases (Sphks) are the rate-limiting kinases in the generation of sphingosine-1-phosphate, which is a well-established intracellular pro-survival lipid mediator. Sphk2 has been reported to be protective following experimental stroke. We investigated the role of Sphk1 in cerebral ischemia using a mouse middle cerebral artery occlusion (MCAO) model and an in vitro glucose-oxygen deprivation (OGD) model. Sphk expression and activity were assessed in the ischemic brain with quantitative PCR (qPCR), western blot, immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Pharmacological and gene knockdown approaches were utilized to investigate the effects of Sphk1 on stroke outcomes. The expression of Sphk1 but not that of Sphk2 was rapidly induced in the cortical penumbra over 96 hours after MCAO, and the microglia were one of the major cellular sources of Sphk1 induction. Consistently, Sphk activity was enhanced in the cortical penumbra. In contrast to the protective role of Sphk2, pharmacological inhibition and cortical knockdown of Sphk1 reduced infarction at 24 and 96 hours after reperfusion. Additionally, the Sphk1 inhibitor improved the neurological deficits at 96 hours after reperfusion. Mechanistically, Sphk1 inhibition and knockdown significantly att...
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research

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ConclusionsThe incidence of PSE after mechanical thrombectomy was low in our cohort. All blood biomarkers displayed interesting sensitivity and specificity. However, the number of PSE cases was small and more studies are needed on risk factors for PSE after mechanical thrombectomy. The potential of blood markers of brain injury markers to contribute to assessment of PSE risk should be explored further.This article is part of the Special Issue "Seizures &Stroke".
Source: Epilepsy and Behavior - Category: Neurology Source Type: research
Source: Neurological Sciences - Category: Neurology Source Type: research
AbstractBackgroundThe Aphasia Rapid Test (ART) is a screening questionnaire used for examining language in acute stroke patients. The ART was initially developed and validated in French. The purpose of this study was to assess the inter-rater reliability of Italian ART.MethodsThe original version of the ART was translated into Italian. The inter-rater reliability was assessed by two independent neurologists who were blind to each other ’s ratings in 52 acute post-stroke patients.ResultsThe 52 patients (28 men, 24 women; mean age 73.73  ± 28.99 years) were included within 1 week of strok...
Source: Neurological Sciences - Category: Neurology Source Type: research
AbstractPosterior circulation strokes affect the vertebrobasilar arterial system, account for about 20 –25% of all ischemic strokes, and are a significant cause of patient disability and mortality. Diagnosis can be challenging; clinical presentation and common pitfalls facing posterior circulation stroke have been discussed elsewhere. In the first part of the review, we focus on the imaging, discus sing the information that can be gathered through a correct selection and interpretation of different possible studies helping to achieve an early diagnosis and to select the best medical treatment. In the second part of t...
Source: Neurological Sciences - Category: Neurology Source Type: research
Publication date: June 2019Source: Revista Portuguesa de Cardiologia (English Edition), Volume 38, Issue 6Author(s): Gonçalo J. Morgado, Inês R. Cruz, Ana Catarina Gomes, Ana Rita Almeida, Maria José Loureiro, Carlos Cotrim, Hélder PereiraAbstractA 24-year-old woman who was 11 weeks pregnant and had a mechanical mitral valve presented at the emergency department with ischemic stroke. Although treatment with aspirin and unfractionated heparin was initially successful, subsequent management was almost impossible, as she eventually suffered a right hemispheric stroke, requiring percutaneous interven...
Source: Revista Portuguesa de Cardiologia - Category: Cardiology Source Type: research
This study aims to analyze the incidence and short-term outcomes associated with stroke (ischemic and hemorrhagic) after implantation of LVAD.MethodsStudy cohorts were identified from the National Inpatient Sample database from January 2009 to September 2015 using ICD 9 codes. The primary outcome was an incidence of stroke and secondary outcomes were associated mortality, length of stay and cost of hospitalization. Multivariate logistic regression analysis was performed to analyze adjusted in-hospital mortality.ResultsUse of LVAD increased significantly from 2009 to 2014 (2,278 in 2009 to 3,730 in 2014 (Ptrend
Source: Indian Heart Journal - Category: Cardiology Source Type: research
AbstractThe poor clinical relevance of experimental models of stroke contributes to the translational failure between preclinical and clinical studies testing anti-inflammatory molecules for ischemic stroke. Here, we (i) describe the time course of inflammatory responses triggered by a thromboembolic model of ischemic stroke and (ii) we examine the efficacy of two clinically tested anti-inflammatory drugs: Minocycline or anti-CD49d antibodies (tested in stroke patients as Natalizumab) administered early (1  h) or late (48 h) after stroke onset. Radiological (lesion volume) and neurological (grip test) outcomes we...
Source: Translational Stroke Research - Category: Neurology Source Type: research
Traumatic cerebral aneurysms are histologically dissecting aneurysms or pseudoaneurysms, thus requiring parent artery occlusion for cure. Combination of endovascular parent artery occlusion and extracranial-intracranial bypass is considered optimal to obtain complete obliteration of the aneurysm and to avoid hemodynamic hypoperfusion. However, endovascular parent artery occlusion of the supraclinoid internal carotid artery (ICA) is at risk of ischemic complications due to distal coil protrusion to adjacent perforating arteries or distal embolism of the thrombi generated in the coil mass.
Source: Journal of Stroke and Cerebrovascular Diseases - Category: Neurology Authors: Tags: Case Report Source Type: research
This study aimed to evaluate the feasibility and safety of thrombolytic therapy administration at Mittaphab Hospital, Lao PDR under the international telestroke consultation system from King Chulalongkorn Memorial Hospital, Thailand. Methods: Acute ischemic stroke patients who presented at Mittaphab Hospital within 4.5 hours after the onset and received thrombolytic therapy between December 2016 and June 2017 were studied.
Source: Journal of Stroke and Cerebrovascular Diseases - Category: Neurology Authors: Source Type: research
Conclusions The presence of comorbidities was linked to NOAC use over vitamin K antagonist, which is different from prescription factor studies in other countries and requires further study.
Source: International Journal of Clinical Pharmacy - Category: Drugs & Pharmacology Source Type: research
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