The Secretome of Apoptotic Human Peripheral Blood Mononuclear Cells Attenuates Secondary Damage Following Spinal Cord Injury in Rats.

This study investigated peripheral blood mononuclear cells as a source of factors for secretome- (MNC-secretome-) based therapy. Specifically, we investigated whether MNC-secretome had therapeutic effects in a rat SCI contusion model and its possible underlying mechanisms. Rats treated with MNC-secretome showed substantially improved functional recovery, attenuated cavity formation, and reduced acute axonal injury compared to control animals. Histological evaluation revealed higher vascular density in the spinal cords of treated animals. Immunohistochemistry showed that MNC-secretome treatment increased the recruitment of CD68(+) cells with concomitant reduction of oxidative stress as reflected by lower expression of inducible nitric oxide synthase. Notably, MNC-secretome showed angiogenic properties ex vivo in aortic rings and spinal cord tissue, and experiments showed that the angiogenic potential of MNC-secretome may be regulated by CXCL-1 upregulation in vivo. Moreover, systemic application of MNC-secretome activated the ERK1/2 pathway in the spinal cord. Taken together, these results indicate that factors in MNC-secretome can mitigate the pathophysiological processes of secondary damage after SCI and improve functional outcomes in rats. PMID: 25797576 [PubMed - as supplied by publisher]
Source: Experimental Neurology - Category: Neurology Authors: Tags: Exp Neurol Source Type: research

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Publication date: Available online 13 September 2019Source: The Veterinary JournalAuthor(s): K.H. Barnes, K.S. Aulakh, C. LiuAbstractManagement of urinary retention after spinal cord injury in dogs is often needed and can include use of medications to relax the urethral sphincter. This was a retrospective study evaluating two such medications, prazosin and diazepam, and whether dogs treated with these medications had different lengths of hospitalization, urinary continence levels, or development of bacteriuria compared to dogs not receiving these medications after thoracolumbar hemilaminectomy for intervertebral disc herni...
Source: The Veterinary Journal - Category: Veterinary Research Source Type: research
Authors: Shackleton C, Evans R, Shamley D, West S, Albertus Y Abstract OBJECTIVES: To evaluate the effectiveness of over-ground robotic locomotor training in individuals with spinal cord injuries with regard to walking performance, cardiovascular demands, secondary health complications and user-satisfaction. DATA SOURCES: PubMed, Cochrane, Web of Science, Scopus, EBSCOhost and Engineering Village. STUDY SELECTION: Trials in which robotic locomotor training was used for a minimum of 3 participants with spinal cord injury. DATA EXTRACTION: Independent extraction of data by 2 reviewers using a pre-establis...
Source: Journal of Rehabilitation Medicine - Category: Rehabilitation Tags: J Rehabil Med Source Type: research
Spinal cord injury is a devastating chronic condition resulting in temporary or permanent motor,sensory or autonomic dysfunction of the cord. The manifestation of spinal cord injury based onthe severity and involved areas could be different. Numerous studies have demonstrated thatbradycardia, hypotension, and orthostatic hypotension are present insignificant number ofpatients after spinal cord injury which peaks at 4th day of injury. Although vasopressors arecommon drugs that have been used to restore blood pressure and heart rate in patients withneurogenic shock, there is limited data regarding pharmacologic management of...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
CONCLUSION: Increased expression of the above molecules was attenuated by both EA and TLR2 antagonism. Our results show that EA attenuates inflammatory pain via TLR2 signalling. PMID: 31517506 [PubMed - as supplied by publisher]
Source: Acupuncture in Medicine : journal of the British Medical Acupuncture Society - Category: Complementary Medicine Authors: Tags: Acupunct Med Source Type: research
Traumatic spinal cord injury can have a dramatic effect on a patient's life. The degree of neurological recovery greatly influences a patient's treatment and expected quality of life. This has resulted in the development of machine learning algorithms (MLA) that use acute demographic and neurological information to prognosticate recovery. The van Middendorp et al. (2011) (vM) logistic regression (LR) model has been established as a reference model for the prediction of walking recovery following spinal cord injury as it has been validated within many different countries.
Source: The Spine Journal - Category: Orthopaedics Authors: Tags: Clinical Study Source Type: research
Condition:   Traumatic Cervical Spinal Cord Injury (CSCI) Intervention:   Other: No interventation Sponsor:   Southeast University, China Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Since the discovery of induced pluripotency more than a decade ago, researchers have been working towards the use of this technology to produce cells for use in tissue engineering and regenerative therapies. Induced pluripotent stem cells are functionally equivalent to embryonic stem cells; given suitable recipes and methods for the surrounding environment and signals, they can be made to generate any of the cell types in the body. The cornea of the eye is a comparatively simple starting point for tissue engineering, easier to work with in many ways, in generating tissues and in delivering cells to the patient. Here, the f...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
Induced pluripotent stem cells (iPSC) [1] have been applied to clinical regenerative cell therapies such as the macular degeneration of the retina [2], spinal cord injury [3] and heart failure [4] et al. As use in autologous settings is hampered by the high time and cost requirements of the individual production of iPSC, an iPSC banking system which collects HLA typed cells from cord blood banks, marrow donor registries and HLA matched platelet donor registries has been proposed [5-8], and tissue transplantation generated from iPSC through banking in allogeneic settings has just been performed [8].
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
Authors: Zhang C, Jing Y, Zhang W, Zhang J, Yang M, Du L, Jia Y, Chen L, Gong H, Li J, Gao F, Liu H, Qin C, Liu C, Wang Y, Shi W, Zhou H, Liu Z, Yang D, Li J Abstract Neurogenic bowel dysfunction (NBD) and gut dysbiosis frequently occur in patients with traumatic cervical spinal cord injury (TCSCI). We evaluated neurogenic bowel management and changes in the gut microbiota in patients with TCSCI as well as associations between these changes and serum biomarkers. Fresh fecal and clinical data were collected from 20 male patients with TCSCI and 23 healthy males. Microbial diversity and composition were analyzed by se...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Abstract Hydrogel matrices with angiogenic properties are much desirable for therapeutic vascularization strategies, namely to provide vascular supply to ischemic areas, transplanted cells, or bioengineered tissues. Here we report the pro-angiogenic effect of fibrin (Fb) functionalization with the T1 sequence from the angiogenic inducer CCN1, forseeing its use in the injured brain and spinal cord. Fibrin functionalization with 40 μM of T1 peptide effectively improved cellular sprouting of human brain microvascular endothelial cells (hCMEC/D3) in the absence of vascular endothelial growth factor (VEGF), withou...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research
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