Silencing LncRNA PVT1 Reverses High Glucose-Induced Regulation of the High Expression of PVT1 in HRMECs by Targeting miR-128-3p
Horm Metab Res 2022; 54: 119-125 DOI: 10.1055/a-1730-5091This paper aims to discuss the possibility of lncRNA PVT1 as a diagnostic
biomarker for diabetic retinopathy (DR) and explore the underlying mechanism.
Real-time quantitative polymerase chain reaction (RT-qPCR) was selected to
determine the expression level of lncRNA PVT1 in the serum of all subjects. The
receiver operating characteristic (ROC) curve reflected the diagnostic
significance of PVT1 for DR patients. The Cell Counting Kit-8 (CCK-8) and
Transwell assays were used to evaluate the effect of PVT1 expression on the
proliferation and migration of human retinal microvascular endothelial cells
(HRMECs). The luciferase reporter gene was selected to verify the interaction
between PVT1 and miR-128-3p. The relative expression level of PVT1 in serum was
higher in both the DB and DR group than in the healthy controls group (HC), and
it was highest in the DR group. ROC curve indicated that serum PVT1 could
distinguish between HC and DB patients, DB patients and DR patients,
respectively. In vitro, high glucose induction significantly increased the
proliferation and migration capabilities of HRMECs, but silencing PVT1 (si-PVT1)
downregulated the proliferation and migration capabilities of HRMECs. The
detection of luciferase reporter gene showed that lncRNA PVT1 targe...
Source: Hormone and Metabolic Research - Category: Endocrinology Authors: Wang, Xuyang Chen, Wangling Lao, Wei Chen, Yunxin Tags: Endocrine Research Source Type: research