An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery

The objective of this chapter is to describe a protocol for culturing SVF on mouse mesentery tissues in order to aid in the discovery of SVF dynamics and associated vessel growth over time. SVF was isolated from the inguinal adipose from adult mice and seeded onto mesentery tissues. Tissues were then cultured for up to 5 days and labeled with endothelial cell and pericyte markers. Representative results demonstrate the observation of SVF-derived vasculogenesis characterized by de novo vessel formation and subsequent vessel connection.PMID:35099735 | DOI:10.1007/978-1-0716-2059-5_12

Source: Mol Biol Cell - January 31, 2022 Category: Molecular Biology Authors: Dima Majbour Ariana D Suarez-Martinez Nicholas A Hodges Arinola O Lampejo Banks M Lomel Elijah W Rice Hulan Shang Adam J Katz Walter L Murfee Source Type: research