Quantification of Leukocyte-Endothelial Cell Interaction During In Vitro Models of Thrombosis at Arterial and Venous Shear Rates

Methods Mol Biol. 2022;2441:287-295. doi: 10.1007/978-1-0716-2059-5_22.ABSTRACTUpon injury, stable thrombi formation requires the recruitment of platelets, leukocytes, and various clotting factors, to provide sufficient inhibition of hemostasis. Classical models of thrombosis involve either ex vivo isolation of platelets and subsequent quantification of aggregation through light transmission aggregometry or in vivo murine intravital thrombosis models (laser injury, ferric chloride, or rose Bengal). Flow adhesion models allow for accurate quantification of the contribution of cell-types to thrombi formation. Here, we describe the use of flow chambers to flow human blood over activated endothelial cells to observe leukocyte-endothelial adhesion at arterial and venous shear rates.PMID:35099745 | DOI:10.1007/978-1-0716-2059-5_22

Source: Mol Biol Cell - January 31, 2022 Category: Molecular Biology Authors: Joshua H Bourne Source Type: research