Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1
The phosphatase domain (PA3346PD) of the response regulator PA3346 modulates the downstream anti-anti-σ factor PA3347 to regulate swarming motility in Pseudomonas aeruginosa PAO1. PA3346PD, which comprises the protein phosphatase 2C domain (PP2C), is classified as a Ser/Thr phosphatase of the Mg2+- or Mn2+-dependent protein phosphatase (PPM) family. The recombinant PA3346PD, with molecular mass 26 kDa, was overexpressed in Escherichia coli, purified on an Ni2+–NTA agarose column and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected from PA3346PD crystals to a resolution of 2.58 Å and the crystals belonged to space group I4132 or I4332, with unit-cell parameter a = 157.61 Å. Preliminary analysis indicates the presence of a monomer of PA3346PD in the asymmetric unit with a solvent content of 58.4%.
Abstract Objectives: The primary target is to reveal whether the resuscitation with hypertonic saline (HTS) or hydroxyethyl starch (HES) would have different effects on the myeloid-derived suppressor cell (MDSC) count and monocytic MDSC (M-MDSC)/granulocytic/neutrophilic MDSC (G-MDSC) rate in the peripheral blood, spleen, and bone marrow nucleated cells (BMNC) in a controlled hemorrhagic shock mouse model under secondary Escherichia coli bacterial infection attack, comparing to resuscitation with normal saline (NS) in 72 hours. Method: After hemorrhagic shock with bacteremia, which is induced by Escherichia c...
Abstract The development of colorectal cancer (CRC) is a multistep process initiated by a benign polyp that has the potential to evolve into in situ carcinoma through the interactions between environmental and genetic factors. CRC incidence rates are constantly increased for young adult patients presenting an advanced tumor stage. The majority of CRCs arise from colonic adenomas originating from aberrant cell proliferation of colon epithelium. Endoscopic polypectomy represents a tool for early detection and removal of polyps, although the occurrence of cancers after negative colonoscopy shows a significant inciden...
We present an uncommon neurological manifestation of rotavirus infection in a 4-year-old Turkish child who presented with hypotonia, reduced consciousness and mutism. Magnetic resonance imaging revealed diffusion abnormalities in the splenium of corpus callosum and nucleus dentatus bilaterally. She was diagnosed with rotavirus cerebellitis. She improved well with dexamethasone and intravenous immunoglobulin but still has dysarthria and poor fine motor coordination. PMID: 32253876 [PubMed - in process]
Conclusions: The study concluded that X-ray exposure showed high effectiveness in treating infected open fractures. PMID: 32256601 [PubMed]
We report the case of a patient who developed a mycotic aortic and ruptured left iliac aneurysm due to E. Coli. The patient developed a secondary aortic graft infection due to a mesenteric ischemia with fecal peritonitis. A literature overview of the current knowledge on mycotic aortic aneurysms specifically due to E. Coli is discussed including the clinical characteristics of patients, the management of the disease and the post-operative outcomes. PMID: 32253984 [PubMed - as supplied by publisher]
Publication date: Available online 7 April 2020Source: Journal of BiotechnologyAuthor(s): Yu Zhou, Mei Zhao, Shenghu Zhou, Yunying Zhao, Guohui Li, Yu Deng
Publication date: Available online 7 April 2020Source: Polymer TestingAuthor(s): Alexander Chubrik, Fedor Senatov, Evgeniy Kolesnikov, Polina Orlova, Maria Poponova, Tatyana Grunina, Mikhail Bartov, Kirill Nikitin, Mikhail Krivozubov, Maria Generalova, Vasily Manskikh, Vladimir Lunin, Alexander Gromov, Anna Karyagina
Volume 11, Issue 1, December 2020, Page 327-336 .
Molecules, Vol. 25, Pages 1711: Composition and Biosynthesis of Scent Compounds from Sterile Flowers of an Ornamental Plant Clematis florida cv. ‘Kaiser’ Molecules doi: 10.3390/molecules25071711 Authors: Yifan Jiang Renjuan Qian Wanbo Zhang Guo Wei Xiaohua Ma Jian Zheng Tobias G. Köllner Feng Chen Clematis florida is a popular ornamental vine species known for diverse colors and shapes of its flowers but not for scent. Here we investigated the composition and biosynthesis of floral scent in ‘Kaiser’, a fragrant cultivar of C. florida that has sterile flowers. Volat...
(American Institute of Physics) Biohybrid robots on the micrometer scale can swim through the body and deliver drugs to tumors or provide other cargo-carrying functions. To be successful, they must consist of materials that can pass through the body's immune response, swim quickly through viscous environments and penetrate tissue cells to deliver cargo. In APL Bioengineering, researchers fabricated biohybrid bacterial microswimmers by combining a genetically engineered E. coli MG1655 substrain and nanoerythrosomes, small structures made from red blood cells.