Organic β‐cyclodextrin Nanoparticle: An Efficient Building Block Between Functionalized Poly(pyrrole) Electrodes and Enzymes

Self-assembly of polystyrene-block- β-cyclodextrin copolymer in water, leads to spherical glyconanoparticles (GNPs). The firm immobilization of GNPs onto electrogenerated poly(pyrrole) films functionalized by adamantane groups is performed by host–guest interactions between β-cyclodextrin and adamantane. GNPs are then used as brid ges for the immobilization of glucose oxidase modified by adamantane groups on polymers. The resulting bioelectrode is applied to glucose detection. AbstractGlyconanoparticles (GNPs) made by self-assembly of carbohydrate-based polystyrene-block- β-cyclodextrin copolymer are used as a building block for the design of nanostructured biomaterials of electrode. The firm immobilization of GNPs is carried out on electrochemically generated polymer, poly(pyrrole-adamantane), and copolymer, poly(pyrrole-adamantane)/poly(pyrrole-lactobionamide) via host–guest interactions between adamantane and β-cyclodextrin. The ability of GNPs for the specific anchoring of biological macromolecules is investigated using glucose oxidase enzyme modified by adamantane groups as a protein model (GOx-Ad). The immobilization of GOx-Ad is carried out by incuba tion of an aqueous enzyme solution on a coating of GNPs adsorbed on a platinum electrode. The presence of immobilized GOx-Ad is evaluated in aqueous glucose solution by potentiostating the underlying platinum electrode at 0.7 V/SCE for the electro-oxidation of H2O2 generated by the enzyme. The analytical performan...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research