Complete genome sequence of a novel mitovirus associated with Lagenaria siceraria

AbstractHere, we report the discovery and molecular characterization of a novel mitovirus isolated from tissues ofLagenaria siceraria, named"Lagenaria siceraria associated mitovirus 1" (LsaMV1). Next-generation sequencing and adapter-ligation-mediated amplification were used to obtain the full-length genome sequence of LsaMV1. The genome of LsaMV1 is 3,098 nucleotides (nt) long and contains an opening reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRP). Homology searches and phylogenetic analysis of the 855-aa RdRP suggested that LsaMV1 is a member of a new species in the familyMitoviridae. This is the first report of a member of the familyMitoviridae associated with the important summer vegetable bottle gourd.

Source: Archives of Virology - December 1, 2021 Category: Virology Source Type: research