Evaluation of HPV Genotyping Assays for Archival Clinical Samples

Human papillomavirus (HPV) testing and genotyping of FFPE tissue samples is important in epidemiological investigations. Here, we compare four different HPV genotyping methods for use in FFPE clinical samples. Comparative testing was performed on 99 samples with a clinical suspicion of HPV. Specimens were analyzed with Anyplex II HPV28 detecting 28 genotypes using real-time PCR and melting curve analysis, CLART HPV2 detecting 35 genotypes using PCR and microarray detection, and MGP5+/6+ consensus primer system together with pyrosequencing.

Source: Journal of Molecular Diagnostics - March 16, 2015 Category: Pathology Authors: Gabriella Lillsunde Larsson, Jessica Carlsson, Mats G. Karlsson, Gisela Helenius Tags: Regular Article Source Type: research