< em > Salmonella enterica < /em > Serovar Diversity, Distribution, and Prevalence in Public Access Waters from a Central California Coastal Leafy Green Growing Region during 2011 - 2016

Appl Environ Microbiol. 2021 Dec 15:AEM0183421. doi: 10.1128/AEM.01834-21. Online ahead of print.ABSTRACTPrevalence and serovar diversity of Salmonella enterica was measured during a five-year survey of surface waters in a 500 mi^2 agricultural region of the Central California Coast. Rivers, streams, lakes, and ponds were sampled bimonthly resulting in 2,979 samples. Overall prevalence was 56.4% with higher levels detected in Spring than in Fall. Small, but significant, differences in prevalence were detected based on sample locations. Detection of Salmonella was correlated positively with both significant rain events and, in some environments, levels of generic Escherichia coli. Analysis of 1,936 isolates revealed significant serovar diversity, with 91 different serovars detected. The most common isolated serovars were S. enterica subsp. enterica serovars I 6,8:d:- (406 isolates, 21.0%, and potentially monophasic Salmonella Muenchen), Give (334 isolates, 17.3%), Muenchen (158 isolates, 8.2%), Typhimurium (227 isolates, 11.7%), Oranienburg (106 isolates, 5.5%), and Montevideo (78 isolates, 4%). Sixteen of the 24 most common serovars detected in the region are among the serovars reported to cause the most human salmonellosis in the United States. Some of the serovars were associated with location and seasonal bias. Analysis of XbaI Pulsed Field Gel Electrophoresis (PFGE) patterns of strains of serovars Typhimurium, Oranienburg, and Montevideo showed significant intra-serovar d...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research