Genetic Variations of Human Papillomavirus Type 16: Implications for Cervical Carcinogenesis.

Genetic Variations of Human Papillomavirus Type 16: Implications for Cervical Carcinogenesis. Jpn J Infect Dis. 2015 Mar 13; Authors: Kukimoto I, Muramatsu M Abstract Human papillomaviruses (HPVs) are the causative agent of cervical cancer, and among approximately 15 high-risk genotypes, HPV16 accounts for more than half the cases of cervical cancer worldwide. Recent progress in determining HPV genomic sequences from clinical samples has revealed a wide variety of HPV16 genome sequences, and has allowed the comprehensive classification of intratype HPV16 variants. These consist of four variant lineages containing nucleotide variations in 1.0-10.0% of the complete viral genome sequences. Epidemiological data suggest that the non-European-Asian lineages of HPV16 entail a higher risk of progression to invasive cervical cancer than the European-Asian lineage. A deep-sequencing analysis has recently demonstrated that the HPV16 genome sequences are highly homogeneous in individual clinical specimens compared with those of RNA viruses. However, an extremely sensitive PCR method, differential DNA denaturation PCR, has detected hypermutation from C to T or G to A in the E2 gene and the long control region of the HPV16 genome, which suggests the involvement of cellular APOBEC proteins in this hypermutation. The quasispecies status of the HPV16 genome in the infected cervix may affect the development of cervical cancer and warrants further inve...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research