Single-strand annealing: Molecular mechanisms and potential applications in CRISPR-Cas-based precision genome editing

CONCLUSION: The SSA mechanism involved in DNA DSB repair appears to be activated by a complex signaling cascade starting with broken end sensing and 5'-3' resection to reveal homologous repeats on the 3' ssDNA overhangs that flank the DSB. Annealing the repeats would help to amend the discontinuous ends and restore the intact genome, resulting in the missing one repeat and the intervening sequence between the repeats. We proposed a model for CRISPR-Cas-based precision insertion or replacement of DNA fragments to take advantage of the characteristics. The proposed model can add a tool to extend the choice for precision gene editing. Nevertheless, the model needs to be experimentally validated and optimized with SSA-favorable conditions for practical applications. This article is protected by copyright. All rights reserved.PMID:34846104 | DOI:10.1002/biot.202100413
Source: Biotechnology Journal - Category: Biotechnology Authors: Source Type: research