GSE189860 Transcriptomic and Epigenomic Profiles of CIC-knockout and IDH1 mutant cells WGBS

Contributors : Stephen D Lee ; Jungeun Song ; Veronique G LeBlanc ; Marco A MarraSeries Type : Methylation profiling by high throughput sequencingOrganism : Homo sapiensCapicua (CIC) ’s transcriptional repressor function is implicated in neurodevelopment and in oligodendroglioma (ODG) aetiology. However, CIC’s role in these contexts remains obscure, primarily from our currently limited knowledge regarding its biological functions. Moreover, CIC mutations in ODG invariably co -occur with a neomorphic IDH1/2 mutation, yet the functional relationship between these two genetic events is unknown. Here, we analysed models derived from an E6/E7/hTERT-immortalized (i.e. p53- and RB-deficient) normal human astrocyte cell line. To examine the consequences of CIC loss, we compared transcriptomic and epigenomic profiles between CIC wildtype and knockout cell lines, with and without mutant IDH1 expression. Our analyses revealed dysregulation of neurodevelopmental genes in association with CIC loss. CIC ChIP-seq was also performed to expand upon the currently limited ensemble o f known CIC target genes. Among the newly identified direct CIC target genes were EPHA2 and ID1, whose functions are linked to neurodevelopment and the tumourigenicity of in vivo glioma tumour models. NFIA, a known mediator of gliogenesis, was discovered to be uniquely overexpressed in double mutant cells (CIC-knockout + IDH1-mutant). These results identify neurodevelopment and specific genes within this...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Homo sapiens Source Type: research

Related Links:

ers Rosendal Korshoej Surgery is essential in the treatment of high-grade gliomas (HGG) and gross total resection (GTR) is known to increase the overall survival and progression-free survival. Several studies have shown that fluorescence-guided surgery with 5-aminolevulinic acid (5-ALA) increases GTR considerably compared to white light surgery (65% vs. 36%). In recent years, sodium fluorescein (SF) has become an increasingly popular agent for fluorescence-guided surgery due to numerous utility benefits compared to 5-ALA, including lower cost, non-toxicity, easy administration during surgery and a wide indication range...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
Prog Brain Res. 2022;268(1):315-327. doi: 10.1016/bs.pbr.2021.10.039. Epub 2021 Dec 14.ABSTRACTGKNS for uveal melanoma has become a recognized valued treatment which avoids enucleation of the eye. In the early days, the eccentric location of these tumors made treatment difficult, but Gamma Knife Perfexion has solved that problem. It is known that larger tumors and tumors with an anterior location have a worse prognosis. GKNS has also been of rather unexpected benefit in optic nerve gliomas that require treatment. Choroidal hemangiomas may benefit from treatment as can secondary glaucoma. It has also been found to be benefi...
Source: Brain Research - Category: Neurology Authors: Source Type: research
Prog Brain Res. 2022;268(1):271-277. doi: 10.1016/bs.pbr.2021.10.036. Epub 2021 Dec 14.ABSTRACTLow grade gliomas concern grade I and grade II tumors. The only grade one tumor is the pilocytic astrocytoma. This is a well-defined tumor with characteristic histology, often accompanied by a cyst. GKNS has been used in surgically inaccessible tumors since 1994. More recently it has been demonstrated that the tumors are more treatment resistant in adults than in children. Grade II tumors include oligodendrogliomas and grade II diffuse astrocytomas. The standard treatment for oligodendrogliomas us total removal followed by fracti...
Source: Brain Research - Category: Neurology Authors: Source Type: research
Prog Brain Res. 2022;268(1):259-270. doi: 10.1016/bs.pbr.2021.10.035. Epub 2021 Dec 14.ABSTRACTGKNS has a limited role in the management of malignant gliomas. It has little or nothing to offer in primary treatment. However, there is evidence that with recurrent tumors which are 4cm in diameter or less, in younger patients, GKNS used together with bevacizumab can prolong overall survival. Nonetheless, more research is required to determine the precise role of the technique.PMID:35074084 | DOI:10.1016/bs.pbr.2021.10.035
Source: Brain Research - Category: Neurology Authors: Source Type: research
CONCLUSION: CfDNA levels showed good diagnostic discrimination in glioma, GBC, breast, lung carcinoma, and OSCC. Significant increase in titers was evident with increase in cancer stage from I to IV in breast, GBC and OSCC.PMID:35074971 | DOI:10.4103/IJPM.IJPM_474_20
Source: Indian Journal of Pathology and Microbiology - Category: Pathology Authors: Source Type: research
J Leukoc Biol. 2022 Jan 24. doi: 10.1002/JLB.5MA1221-468R. Online ahead of print.ABSTRACTPolypeptide N-acetylgalactosaminyltransferases (GalNAc-Ts), a group of isoenzymes that initiate mucin-type O-glycosylation, have been shown to mediate tumor growth and metastasis in various cancer types. However, data on the clinical significance and features of GalNAc-Ts remain scant. Here, we used Oncomine and The Cancer Genome Atlas (TCGA) databases to analyze the transcription and survival effect of GALNTs (N-acetylgalactosaminyltransferase genes) in pan-cancer. The data showed that the GALNTs were aberrantly expressed in various h...
Source: Journal of Leukocyte Biology - Category: Hematology Authors: Source Type: research
Nat Prod Res. 2022 Jan 25:1-5. doi: 10.1080/14786419.2021.2017928. Online ahead of print.ABSTRACTIn this work, four alkaloids from the stem bark of T. catharinensis were isolated, namely: voacangine (1); ethyl apovincaminate (2); affinisine (3) and voachalotine (4). The alkaloids were tested in vitro for antiproliferative capacity in eight tumor cell lines: U251 (glioma), MCF-7 (breast), NCI-ADR/RES (drug resistant ovary), 786-0 (kidney), NCI-H460 (lung), HT-29 (colon), K562 (leukemia) and PC-3 (prostate) and a non-tumor keratinocyte cell line (HaCat). Antiproliferative activity was observed after 48 hours and results expr...
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research
In conclusion, KIAA0101 silencing inhibited glioma progression and glycolysis by inactivating the PI3K/AKT/mTOR pathway.
Source: Metabolic Brain Disease - Category: Neurology Source Type: research
ConclusionThe use of synthetic vascular grafts as a means of stenting slit valves is a safe and effective novel treatment option for high-pressure tumor bed cysts.
Source: Acta Neurochirurgica - Category: Neurosurgery Source Type: research
ConclusionsThis is the first report of clinical outcomes of panobinostat in adults with H3 K27M-mutant DMG. We showed that it is well-tolerated at the dosage schedule that we describe, with no serious adverse effects throughout the study period.
Source: Journal of Neuro-Oncology - Category: Cancer & Oncology Source Type: research
More News: Brain | Brain Tumor | Genetics | Glioma | Neurology