The Evolving Direct and Indirect platforms for the Detection of SARS-CoV-2

J Virol Methods. 2021 Nov 26:114381. doi: 10.1016/j.jviromet.2021.114381. Online ahead of print.ABSTRACTDiagnosis of SARS-CoV-2 by standard screening measures can reduce the chance of COVID-19 spread before the symptoms become severe. Detecting viral RNA and antigens, anti-viral antibodies, and CT-scan are the most routine diagnostic methods. Accordingly, several diagnostic platforms including thermal and isothermal amplifications, CRISPR/Cas‑based approaches, digital PCR, ELISA, NGS, and point-of-care testing methods with variable sensitivities, have been developed that may facilitate managing and preventing the further spread of the infection. Here, we summarized the currently available direct and indirect testing platforms in research and clinical settings, including recent progress in the methods to detect viral RNA, antigens, and specific antibodies. This summary may help in selecting the effective method for a special application sucha as routine laboratory diagnosis, point-of-care tests or tracing the the virus spread and mutations.PMID:34843826 | PMC:PMC8626143 | DOI:10.1016/j.jviromet.2021.114381
Source: Journal of Virological Methods - Category: Virology Authors: Source Type: research