Measurement methods of single cell drug response

Talanta. 2021 Nov 3:123035. doi: 10.1016/j.talanta.2021.123035. Online ahead of print.ABSTRACTIn the last decades, a wide multitude of research activity has been focused on the development of new drugs, and devoted to overcome the challenges of high cost and low efficiency in drug evaluation. The measurement of drug response at the single cell level is a quicker, more direct and more accurate way to reflect drug efficacy, which can shorten the drug development period and reduce research costs. Therefore, the single cell drug response (SCDR) measurement technology has aroused extensive attention from researchers, and has become a hot topic in the fields of drug research and cell biology. Recent years have seen the emergence of various SCDR measurement technologies that feature different working principles and different levels of measurement performance. To better examine, compare and summarize the characteristics and functions of these technologies, we select signal-to-noise ratio, throughput, content, invasion, and device complexity as the criteria to evaluate them from the drug efficacy perspective. This review aims to highlight sixteen kinds of SCDR measurement technologies, including patch-clamp technique, live-cell interferometry, capillary electrophoresis, secondary ion mass spectrometry, and more, and report widespread representative examples of SCDR measurement the recent approaches for over the past forty years. Based on their reaction principles, these technologies a...

Source: Talanta - November 29, 2021 Category: Chemistry Authors: Yuliang Zhao Jiazhi Lou Hongyu Zhang Hui Sun Menglin Zhang Shuyu Wang Xiaopeng Sha Zhikun Zhan Ying Wang Cuihua Ma Wen Jung Li Source Type: research