Genes, Vol. 12, Pages 1913: The Effects of Sequence Length and Composition of Random Sequence Peptides on the Growth of E. coli Cells

Genes, Vol. 12, Pages 1913: The Effects of Sequence Length and Composition of Random Sequence Peptides on the Growth of E. coli Cells Genes doi: 10.3390/genes12121913 Authors: Johana Fajardo Diethard Tautz We study the potential for the de novo evolution of genes from random nucleotide sequences using libraries of E. coli expressing random sequence peptides. We assess the effects of such peptides on cell growth by monitoring frequency changes in individual clones in a complex library through four serial passages. Using a new analysis pipeline that allows the tracing of peptides of all lengths, we find that over half of the peptides have consistent effects on cell growth. Across nine different experiments, around 16% of clones increase in frequency and 36% decrease, with some variation between individual experiments. Shorter peptides (8–20 residues), are more likely to increase in frequency, longer ones are more likely to decrease. GC content, amino acid composition, intrinsic disorder, and aggregation propensity show slightly different patterns between peptide groups. Sequences that increase in frequency tend to be more disordered with lower aggregation propensity. This coincides with the observation that young genes with more disordered structures are better tolerated in genomes. Our data indicate that random sequences can be a source of evolutionary innovation, since a large fraction of them are well tolerated by the cells or can provide a growth adv...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research