Accumulation of radio-iron and plutonium, alone and in combination, in Pseudomonas putida grown in liquid cultures
The impact of low doses of ionising radiation on biological and environmental systems have been
historically difficult to study. Modern biological tools have provided new methods for studying
these mechanisms but applying these tools to a dose –response relationship may require refinement of
dosimetric techniques that incorporate a detailed understand of radionuclide accumulation in
biological cells, particularly when assessing the impact of low doses of ionising radiation. In this
work Pseudomonas putida ( KT2440) grown in liquid culture was exposed to low dose rates (10 –20 mGy d
−1 ) of 239 Pu and 55 Fe, both alone and in combination, for a period of 20 days, and the
accumulation of 239 Pu and 55 Fe in cell pellets was analysed via liquid scintillation counting. The
study also considered of cells grown with 239 Pu and stable Fe (primarily 56 Fe). In addition to the
analysis of cell pellet and media sampl...
Source: Journal of Radiological Protection - Category: Physics Authors: Lisa M Manglass, Molly Wintenberg, Charlotte Vogel, Mark Blenner and Nicole E Martinez Source Type: research