Proteomic profiling of cryopreserved Trichormus variabilis using various cryoprotectants

Cryobiology. 2021 Nov 19:S0011-2240(21)00350-3. doi: 10.1016/j.cryobiol.2021.11.175. Online ahead of print.ABSTRACTAlgae, which may be unicellular or multicellular, can carry out photosynthesis just like plants as they effectively utilize light energy. They contain various physiologically active substances and are, therefore, widely used commercially to produce healthy food and feed additives, cosmetics, and energy supplements. For useful applications, the cryopreservation technique has been used in various fields. Recently, to develop suitable cryopreservation methods for algal applications, various studies have been performed. However, adequate investigations have not been conducted to understand the mechanism underlying algal cryopreservation at the molecular level. Therefore, this study examined the profile alteration of the proteome using cryopreservation with various cryoprotectants (CPAs). Trichormus variabilis was cultured and then cryopreserved with 10% dimethyl sulfoxide, methanol, and glycerol, after which, proteome profiling was done. Finally, signaling pathway search was performed, and a new signaling pathway was established based on differentially expressed proteins. As a result, the expression levels of 17 proteins were observed. Additionally, it was confirmed that the differentially expressed proteins were related to 16 signaling pathways and that they were capable of interacting with each other. The findings suggest that the differentially expressed proteins ...

Source: Cryobiology - November 22, 2021 Category: Biology Authors: Jeong-Won Bae Mirye Park Chang Soo Lee Woo-Sung Kwon Source Type: research