Progression of diabetes is associated with changes in the ileal transcriptome and ileal ‐colon morphology in the UC Davis Type 2 Diabetes Mellitus rat

The advancement of diabetes in the UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) rat model results in a trophic effect on the mucosal epithelia. Diabetes was also associated with regulation of gastrointestinal tract RNA expression, which appears more pronounced in the ileum relative to the colon. The UCD-T2DM rat spontaneously develops diabetes while consuming standard chow, thus, these changes may be diabetes specific. AbstractDeterioration in glucose homeostasis has been associated with intestinal dysbiosis, but it is not known how metabolic dysregulation alters the gastrointestinal environment. We investigated how the progression of diabetes alters ileal and colonic epithelial mucosal structure, microbial abundance, and transcript expression in the University of California Davis Type 2 Diabetes Mellitus (UCD-T2DM) rat model. Male UCD-T2DM rats (age ~170  days) were included if<1-month (n = 6, D1M) or 3-month (n = 6, D3M) post-onset of diabetes. Younger nondiabetic UCD-T2DM rats were included as a nondiabetic comparison (n = 6, ND, age ~70 days). Ileum villi height/crypt depths and colon crypt depths were assessed by histology. Microbial abundance of colon content was measured with 16S rRNA sequencing. Ileum and colon transcriptional abundances were analyzed using RNA sequencing. Ileum villi height and crypt depth were greater in D3M rats compared to ND. Colon crypt depth was greatest in D3M rats compared to both ND and D1M rats. Colon abundances ofAkkermansia and...
Source: Physiological Reports - Category: Physiology Authors: Tags: ORIGINAL ARTICLE Source Type: research