Slide over: advances in slide-free optical microscopy as drivers of diagnostic pathology

Am J Pathol. 2021 Nov 10:S0002-9440(21)00471-5. doi: 10.1016/j.ajpath.2021.10.010. Online ahead of print.ABSTRACTConventional clinical histopathology analysis is based on bright-field microscopy of thinly sliced tissue specimens. Although bright-field microscopy is a simple and robust method for examining microscope slides, preparing the slides needed is a lengthy and labor-intensive process. Slide-free histopathology technologies address this problem by directly imaging intact, minimally processed tissue samples using advanced optical imaging systems, bypassing the extended workflow now required for preparation of tissue sections. This manuscript explains the technical basis of slide-free microscopy, reviews common slide-free optical microscopy techniques, and discusses the opportunities and challenges involved in clinical implementation.PMID:34774514 | DOI:10.1016/j.ajpath.2021.10.010

Source: The American Journal of Pathology - November 14, 2021 Category: Pathology Authors: Yehe Liu Richard M Levenson Michael W Jenkins Source Type: research