Granulosa cell proliferation in the gilt ovary associated with ovarian status and porcine reproductive and respiratory syndrome virus detection

Abstract The aims of the present study were to quantify the number of follicles and determine granulosa cell proliferation in the ovaries of gilts in relation to ovarian status and porcine reproductive and respiratory syndrome (PRRS) virus infection. Ovarian tissues were obtained from 37 Landrace × Yorkshire crossbred gilts aged 270.7 ± 24.5 days and weighing 144.5 ± 15.0 kg (27 cycling and 10 prepubertal gilts). PRRS virus was detected using immunohistochemistry, and the ovaries were classified as negative (n = 17) or positive (n = 20) to PRRS virus. Granulosa cell proliferation was determined by proliferating cell nuclear antigen (PCNA) immunostaining. The proportion of PCNA-expressing granulosa cells in pre-antral (n = 197) and antral follicles (n = 76) was determined using Image-Pro® Plus software. The number of follicles in ovarian tissue containing PRRS virus did not differ significantly compared with those without PRRS virus. Granulosa cell proliferation was influenced by the type of follicle (P 
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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AbstractArtificial intelligence (AI) has been proposed as a potential tool to help address many of the existing problems related with empirical or subjective assessments of clinical and embryological decision points during the treatment of infertility. AI technologies are reviewed and potential areas of implementation of algorithms are discussed, highlighting the importance of following a proper path for the development and validation of algorithms, including regulatory requirements, and the need for ecosystems containing enough quality data to generate it. As evidenced by the consensus of a group of experts in fertility i...
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
AbstractImproving reproductive performance of repeat breeder buffalo cows due to clinical endometritis is crucial in overcoming infertility problems in buffalo cows. The aim of the present study was to use PGF2 α and/or gentamicin 10% for treatment of endometritis and to determine biochemical parameters in serum that could be used to diagnose endometritis in buffalo cows. A total of 64 anestrous buffalo cows were assigned into one of five treatment groups: group one (n = 9) buffalo cows were physiological normal cows and served as a control group; group 2 buffalo cows had endometritis, but were not trea...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
Source: Human Fertility - Category: Reproduction Medicine Authors: Source Type: research
Source: Human Fertility - Category: Reproduction Medicine Authors: Source Type: research
ConclusionsThese recommendations could set the basis for a public health action plan for primary management of reproductive health. The findings may be applicable to any country whose health services system provides primary healthcare.
Source: Irish Journal of Medical Science - Category: General Medicine Source Type: research
In conclusion, it remains unclear if brain-specific regional and temporal changes occur in the expression of the different APP variants during AD progression. Since APP is also found in blood cells, assessing the changes in APP mRNA expression in peripheral blood cells from AD patients has been considering an alternative. However, again the quantification of APP mRNA in peripheral blood cells has generated controversial results. Brain APP protein has been analyzed in only a few studies, probably as it is difficult to interpret the complex pattern of APP variants and fragments. We previously characterized the soluabl...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
ConclusionsThis study demonstrates an objective observation of sleep time reduction in women with subfertility, although it is not yet clear if this association is casual. Given our increased understanding of the internal body clock and circadian rhythm on fertility, our observation warrants further investigation.
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
CONCLUSION: In the short term, this MBP did not affect weight loss in infertile women, but the MBP intervention contributed to reduce waist circumference, possibly due to a significant decrease in food energy intake. TRIAL REGISTRATION NUMBER: RBR-7by76r. PMID: 32972271 [PubMed - as supplied by publisher]
Source: Journal of Psychosomatic Obstetrics and Gynaecology - Category: OBGYN Tags: J Psychosom Obstet Gynaecol Source Type: research
Selective estrogen receptor modulators (SERMs) are an increasingly important therapeutic modality that are used by clinicians on a daily basis. Unfortunately, clinicians have a limited understanding regarding the underlying mechanism(s) of how SERMs function and their increasingly useful role in the treatment of estrogen-responsive target tissues such as the breast, bone, vagina, uterine endometrium, and brain. This review will provide a basic understanding of our current knowledge of SERM pharmacodynamics and will highlight the clinical applications of Food and Drug Administration–approved SERMs in the treatment of ...
Source: Menopause - Category: OBGYN Tags: Clinical Corner: Invited Review Source Type: research
AbstractAutoimmune thyroid disease is the most common endocrine disorder during pregnancy. Thyroid autoantibodies (TAs) have been suggested to serve a role in implantation failure and spontaneous abortion. Until now, there are no data on the potential interaction of TAs with human reproductive organs. Here, we set out for the first time to test this hypothesis by studying the expression of thyroid peroxidase (TPO) at gene and protein level in human reproductive organs. Endometrial samples were taken from normal women, and placenta tissues were collected after full-term caesarian section. Expression of TPO messenger RNA (mR...
Source: Clinical and Experimental Medicine - Category: Research Source Type: research
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