Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin Against Daptomycin-Nonsusceptible < em > Enterococcus faecium < /em >

Antimicrob Agents Chemother. 2021 Nov 1:AAC0162321. doi: 10.1128/AAC.01623-21. Online ahead of print.ABSTRACTEnterococcus faecium(E. fcm) is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. fcm strains against 4 phages, two phages(113, 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin(DAP) plus phage cocktail(113:myophage;9184:siphopage) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin non-susceptible E. fcm.PMID:34723631 | DOI:10.1128/AAC.01623-21

Source: Antimicrobial Agents and Chemotherapy - November 1, 2021 Category: Microbiology Authors: Taylor Morrisette Katherine L Lev Gregory S Canfield Breck A Duerkop Razieh Kebriaei Kyle C Stamper Dana Holger Susan M Lehman Smaranda Willcox Cesar A Arias Michael J Rybak Source Type: research