Evaluation of Bacteriophage Cocktails Alone and in Combination with Daptomycin Against Daptomycin-Nonsusceptible Enterococcus faecium

Antimicrob Agents Chemother. 2021 Nov 1:AAC0162321. doi: 10.1128/AAC.01623-21. Online ahead of print.ABSTRACTEnterococcus faecium(E. fcm) is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. fcm strains against 4 phages, two phages(113, 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin(DAP) plus phage cocktail(113:myophage;9184:siphopage) showed bactericidal activity in most regimens, while DAP addition prevented phage 9184 resistance against daptomycin non-susceptible E. fcm.PMID:34723631 | DOI:10.1128/AAC.01623-21
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Source Type: research

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CONCLUSION: Further studies on bacteriophages and their efficacy on enterococcal strains could increase the treatment possibility of enterococcal infections. Due to these bacteriophages' effects on Streptococcus strains, bacteriophages may be used to treat streptococcal infections as well.PMID:34900165 | PMC:PMC8629828 | DOI:10.18502/ijm.v13i5.7434
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Antimicrob Agents Chemother. 2021 Nov 1:AAC0162321. doi: 10.1128/AAC.01623-21. Online ahead of print.ABSTRACTEnterococcus faecium(E. fcm) is a significant multidrug-resistant pathogen. Bacteriophage cocktails are being proposed to complement antibiotic therapy. After a screen of 8 E. fcm strains against 4 phages, two phages(113, 9184) with the broadest host ranges were chosen for further experiments. Transmission electron microscopy, whole-genome sequencing, comparative genome analyses, and time-kill analyses were performed. Daptomycin(DAP) plus phage cocktail(113:myophage;9184:siphopage) showed bactericidal activity in mo...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Source Type: research
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