Architectural and functional details of CF IA proteins involved in yeast 3'-end pre-mRNA processing and its significance for eukaryotes: A concise review

Int J Biol Macromol. 2021 Oct 23:S0141-8130(21)02287-X. doi: 10.1016/j.ijbiomac.2021.10.129. Online ahead of print.ABSTRACTIn eukaryotes, maturation of pre-mRNA relies on its precise 3'-end processing. This processing involves co-transcriptional steps regulated by sequence elements and other proteins. Although, it holds tremendous importance, defect in the processing machinery will result in erroneous pre-mRNA maturation leading to defective translation. Remarkably, more than 20 proteins in humans and yeast share homology and execute this processing. The defects in this processing are associated with various diseases in humans. We shed light on the CF IA subunit of yeast Saccharomyces cerevisiae that contains four proteins (Pcf11, Clp1, Rna14 and Rna15) involved in this processing. Structural details of various domains of CF IA and their roles during 3'-end processing, like cleavage and polyadenylation at 3'-UTR of pre-mRNA and other cellular events are explained. Further, the chronological development and important discoveries associated with 3'-end processing are summarized. Moreover, the mammalian homologues of yeast CF IA proteins, along with their key roles are described. This knowledge would be helpful for better comprehension of the mechanism associated with this marvel; thus opening up vast avenues in this area.PMID:34699898 | DOI:10.1016/j.ijbiomac.2021.10.129

Source: International Journal of Biological Macromolecules - October 26, 2021 Category: Biochemistry Authors: Mandeep Kaur Akanksha Sharma Gurpal Singh Santosh Kumar Ravi P Barnwal Source Type: research