Gene Expression Analysis with No Sequence Data: Study on Reeves's Muntjac ( < em > Muntiacus reevesi < /em > )

In this study, we attempt to design primers for a real-time PCR analysis, basing on a comparative analysis of selected gene sequences of species related to Reeves's muntjac (Muntiacus reevesi) and by identifying highly conservative regions. Results of PCR products sequencing and their alignment with the GenBank collection show that all selected primers gave products highly similar (> 90%) to the intended target (among compared species), which led us to the conclusion that our primers may be used for further analyses of gene expression.PMID:34698093 | DOI:10.3390/cimb43030111
Source: Current Issues in Molecular Biology - Category: Molecular Biology Authors: Source Type: research