Scientific and Regulatory Policy Committee Points to Consider: Fixation, Trimming, and Sectioning of Nonrodent Eyes and Ocular Tissues for Examination in Ocular and General Toxicity Studies

Toxicol Pathol. 2021 Oct 25:1926233211047562. doi: 10.1177/01926233211047562. Online ahead of print.ABSTRACTA Working Group of the Society of Toxicologic Pathology's Scientific and Regulatory Policy Committee conducted a technical and scientific review of current practices relating to the fixation, trimming, and sectioning of the nonrodent eye to identify key points and species-specific anatomical landmarks to consider when preparing and evaluating eyes of rabbits, dogs, minipigs, and nonhuman primates from ocular and general toxicity studies. The topics addressed in this Points to Consider article include determination of situations when more comprehensive evaluation of the globe and/or associated extraocular tissues should be implemented (expanded ocular sampling), and what constitutes expanded ocular sampling. In addition, this manuscript highlights the practical aspects of fixing, trimming, and sectioning the eye to ensure adequate histopathological evaluation of all major ocular structures, including the cone-dense areas (visual streak/macula/fovea) of the retina for rabbits, dogs, minipigs, and nonhuman primates, which is a current regulatory expectation for ocular toxicity studies.[Box: see text].PMID:34693851 | DOI:10.1177/01926233211047562

Source: Toxicologic Pathology - October 25, 2021 Category: Pathology Authors: Helen S Booler Typhaine Lejeune Steven Sorden Margarita M Gruebbel Kenneth A Schafer Brian Short Cindy Farman Meg Ferrell Ramos Bindu Bennet Krishna Yekkala Elke-Astrid Atzpodien Oliver C Turner Jaqueline Brassard George Foley Source Type: research