From a dimer to a monomer: Construction of a chimeric monomeric isocitrate dehydrogenase

In this study, a chimeric monomeric IDH was successfully constructed by using homo-dimericEscherichia coli IDH as a start point by rational design and site-saturation mutagenesis. The ~67  kDa chimeric enzyme behaved as a monomer in solution, with aKm of 61  μM and akcat of 15  s−1 for isocitrate in the presence of NADP+ and Mn2+. Our result demonstrated that dimeric IDHs have a potential to evolve monomeric ones. The evolution of the IDH family was also discussed.
Source: Protein Science - Category: Biochemistry Authors: Tags: Full ‐Length Paper Source Type: research

Related Links:

Chang Won Choi A Korean isolate of the sacbrood virus infecting Apis cerana (AcSBV-Kor) is the most destructive honeybee virus, causing serious economic damage losses in Korean apiculture. To address this, here, we attempted to develop an assay for the rapid detection of AcSBV-Kor based on immunochromatographic detection of constituent viral proteins. Genes encoding VP1 and VP2 proteins of AcSBV-Kor were cloned into an expression vector (pET-28a) and expressed in Escherichia coli BL21(DE3). During purification, recombinant VP1 (rVP1) and VP2 (rVP2) proteins were found in the insoluble fraction, with a molecular size...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
AbstractCharacteristics and quality aspects of milk from native ovine Queue Fine de l ’Ouest (QFO) and the exotic D’man (DMN) breeds were investigated and compared with those of two subgroups of the local goat population Arbi and Serti. A total of 418 individual milk samples were collected from multiparous lactating animals reared in the continental oasis region of Tunisia. Sampl es were analyzed for physical parameters (pH, density, and acidity), chemical composition (dry matter, fat, protein, lactose, casein, ash, and casein/protein ratio), mineral concentrations (Ca, P, Na, K, and Ca/P) and microbiological...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
SPRING HOUSE, PENNSYLVANIA, December 3, 2021 – The Janssen Pharmaceutical Companies of Johnson &Johnson today announced new TREMFYA® (guselkumab) efficacy and safety data from the Phase 3b COSMOS trial published in Annals of the Rheumatic Diseases (ARD), evaluating this selective interleukin (IL)-23 inhibitor in adults with active psoriatic arthritis (PsA) who demonstrated inadequate efficacy or intolerance to tumor necrosis factor inhibition (TNFi).1 Results showed significantly higher proportions of patients treated with TREMFYA had improvement in joint signs and symptoms and complete skin clearance versus ...
Source: Johnson and Johnson - Category: Pharmaceuticals Tags: Innovation Source Type: news
This study was aimed to investigate the difference in laboratory parameters between Febrile Seizure and control groups.PATIENTS AND METHODS: In this study, 169 children admitted to the pediatric emergency department with their first episode of FS and 189 control groups were retrospectively analyzed. The demographic characteristics and laboratory parameters of children were obtained from their files.RESULTS: Upper respiratory tract infection (URTI) was determined the most common disease (81.6%) in the FC group followed by acute gastroenteritis (AGE) (15.4%) and urinary tract infection (UTI) (3%), respectively. Similarly, UR...
Source: Pharmacological Reviews - Category: Drugs & Pharmacology Authors: Source Type: research
In this study, we describe two enzymatic cascades to synthesize AICA derivatives with defined phosphorylation levels from the corresponding nucleobase and the co-substrate phosphoribosyl pyrophosphate. The cascades are composed of an adenine phosphoribosyltransferase from Escherichia coli (EcAPT) and different polyphosphate kinases: polyphosphate kinase from Acinetobacter johnsonii. (AjPPK) and polyphosphate kinase from Meiothermus ruber (MrPPK). The role of the EcAPT is to bind the nucleobase to the sugar moiety, while the kinases are responsible for further phosphorylation of the nucleotide to produce the desired phospho...
Source: Chembiochem - Category: Biochemistry Authors: Source Type: research
CONCLUSION: The study documents the frequency of clinically significant anaerobic bacteria in various infections, and their associations with aerobes in polymicrobial growth. The present study may aid us in devising better therapeutic strategies against both aerobes and anaerobes in anaerobic infections, which is often empirical. Besides, the data can update clinicians with the changing patterns of anaerobic infections, which remains a neglected concern.PMID:34861364 | DOI:10.1016/j.anaerobe.2021.102482
Source: Anaerobe - Category: Microbiology Authors: Source Type: research
This study was aimed to investigate the difference in laboratory parameters between Febrile Seizure and control groups.PATIENTS AND METHODS: In this study, 169 children admitted to the pediatric emergency department with their first episode of FS and 189 control groups were retrospectively analyzed. The demographic characteristics and laboratory parameters of children were obtained from their files.RESULTS: Upper respiratory tract infection (URTI) was determined the most common disease (81.6%) in the FC group followed by acute gastroenteritis (AGE) (15.4%) and urinary tract infection (UTI) (3%), respectively. Similarly, UR...
Source: Pharmacological Reviews - Category: Drugs & Pharmacology Authors: Source Type: research
Chemosphere. 2021 Nov 30:133125. doi: 10.1016/j.chemosphere.2021.133125. Online ahead of print.ABSTRACTOrganophosphate flame retardants (OPFRs) are emerging environmental pollutants that are increasingly being used in consumer commodities. The adverse effects on biota induced by tris(2-chloroethyl) phosphate (TCEP) and triphenyl phosphate (TPHP) have become a growing concern. Unfortunately, toxic mechanisms at the molecular level for OPFRs in organisms are still lacking. Herein, Escherichia coli (E.coli) was exposed to TCEP and TPHP for 24 and 48 h to reveal oxidative stress response and molecular toxicity mechanisms. The ...
Source: Chemosphere - Category: Chemistry Authors: Source Type: research
This study was aimed to investigate the difference in laboratory parameters between Febrile Seizure and control groups.PATIENTS AND METHODS: In this study, 169 children admitted to the pediatric emergency department with their first episode of FS and 189 control groups were retrospectively analyzed. The demographic characteristics and laboratory parameters of children were obtained from their files.RESULTS: Upper respiratory tract infection (URTI) was determined the most common disease (81.6%) in the FC group followed by acute gastroenteritis (AGE) (15.4%) and urinary tract infection (UTI) (3%), respectively. Similarly, UR...
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Authors: Source Type: research
In this study, a sample pretreatment protocol and a flow cytometry method were developed for rapid and multiplexed quantification of the three pathogens without cultural enrichment in ground beef. The whole process of sample pretreatment, staining, and instrument analysis can be accomplished within 1 h. The three bacteria upon sample pretreatment were demonstrated good recoveries (93.8%-101.2%). The quantitative detection range of the mothed was 103 to 108 cells/g for all three pathogens, and the detection limit for Salmonella, E. coli O157:H7 and S. flexneri in ground beef were 3.1 × 103 cells/g, 2.1 × 103 cel...
Source: Talanta - Category: Chemistry Authors: Source Type: research
More News: Biochemistry | Gastroenteritis | Genetics | Laboratory Medicine | Science | Study