Spt20, a structural subunit of the SAGA complex, regulates biofilm formation, asexual development, and virulence of < em > Aspergillus fumigatus < /em >

In this study, Spt20, an orthologue of the subunit of Saccharomyces cerevisiae transcriptional co-activator Spt-Ada-Gcn5-acetyltransferase (SAGA) complex, was identified as a regulator of biofilm formation and asexual development in A. fumigatus. The loss of spt20 caused severe defects in GAG biosynthesis, biofilm formation, conidiation, and virulence of A. fumigatus. RNA-sequence data demonstrated that Spt20 positively regulates the expression of GAG biosynthesis genes uge3 and agd3, developmental regulator medA, and genes involved in the conidiation pathway. Moreover, more than 10 subunits of the SAGA complex (known from yeast) could be immunoprecipitated with Spt20, suggesting that Spt20 acts as a structural subunit of the SAGA complex. Furthermore, distinct modules of SAGA regulate GAG biosynthesis, biofilm formation, and asexual development in A. fumigatus to varying degrees. In summary, the novel biofilm regulator Spt20 is reported, which plays a crucial role in the regulation of fungal asexual development, GAG biosynthesis, and virulence of A. fumigatus. These findings expand knowledge on the regulatory circuits of the SAGA complex relevant for biofilm formation and asexual development of A. fumigatus. IMPORTANCE Eukaryotic transcription is regulated by a large number of proteins, ranging from sequence-specific DNA binding factors to transcriptional co-activators (chromatin regulators and the general transcription machinery) and their regulators. Previous research indi...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research