Genes, Vol. 12, Pages 1657: Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon

Genes, Vol. 12, Pages 1657: Genetic Diversity of Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) in Field Isolates from Five Different Areas of the Brazilian Amazon Genes doi: 10.3390/genes12111657 Authors: Lana Bitencourt Chaves Glaucia de Oliveira Guimarães Daiana de Souza Perce-da-Silva Dalma Maria Banic Paulo Renato Rivas Totino Ricardo Luiz Dantas Machado Rodrigo Nunes Rodrigues-da-Silva Lilian Rose Pratt-Riccio Cláudio Tadeu Daniel-Ribeiro Josué da Costa Lima-Junior The Plasmodium vivax Cysteine-Rich Protective Antigen (PvCyRPA) has an important role in erythrocyte invasion and has been considered a target for vivax malaria vaccine development. Nonetheless, its genetic diversity remains uncharted in Brazilian malaria-endemic areas. Therefore, we investigated the pvcyrpa genetic polymorphism in 98 field isolates from the Brazilian Amazon and its impact on the antigenicity of predicted B-cell epitopes. Genetic diversity parameters, population genetic analysis, neutrality test and the median-joining network were analyzed, and the potential amino acid polymorphism participation in B-cell epitopes was investigated. One synonymous and 26 non-synonymous substitutions defined fifty haplotypes. The nucleotide diversity and Tajima’s D values varied across the coding gene. The exon-1 sequence had greater diversity than those of exon-2. Concerning the prediction analysis, seven sequences were predicted as linear B cell epitopes, the majori...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research