< em > Coprinopsis cinerea < /em > uses laccase Lcc9 as a defense strategy to eliminate oxidative stress during fungal-fungal interactions

Appl Environ Microbiol. 2021 Oct 20:AEM0176021. doi: 10.1128/AEM.01760-21. Online ahead of print.ABSTRACTFrequently, laccases are triggered during fungal cocultivation for overexpression. The function of these activated laccases during coculture has not been clarified. Previously, we reported that Gongronella sp. w5 (w5) (Mucoromycota, Mucoromycetes) specifically triggered the laccase Lcc9 overexpression in Coprinopsis cinerea (Basidiomycota, Agaricomycetes). To systematically analyze the function of the overexpressed laccase during fungal interaction, C. cinerea mycelia before and after the initial Lcc9 overexpression were chosen for transcriptome analysis. Results showed that accompanied by specific utilization of fructose as carbohydrate substrate, oxidative stress derived from antagonistic compounds secreted by w5 appears to be a signal critical for laccase production in C. cinerea. Reactive oxygen species (ROS) decrease in the C. cinerea wild-type strain followed the increase in laccase production and then, lcc9 transcription and laccase activity stopped. By comparison, increased H2O2 content and mycelial ROS levels were observed during the entire cocultivation in lcc9 silenced C. cinerea strains. Moreover, lcc9 silencing slowed down the C. cinerea mycelial growth, affected hyphal morphology, and decreased the asexual sporulation in coculture. Our results showed that intracellular ROS acted as signal molecules to stimulate defense responses by C. cinerea with the express...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research