Photoactivated Self ‐Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low‐Dose Photodynamic Therapy

An efficient autophagy suppressor is constructed for improving the therapeutic effect of low-dose photodynamic therapy, which can perform photoactivated burst release of oligonucleotide drug by self-disassembly of DNA nanostructure to achieve amplifiedATG5 gene silencing for enhancing antitumor effect. AbstractLow-dose photodynamic therapy (PDT) holds great promise for reducing undesired patient photosensitivity in cancer treatment. Yet, its therapeutic effect is significantly affected by intracellular cytoprotective processes, such as autophagy. Here, an efficient autophagy suppressor is developed, which is a multifunctional DNA nanoflower (DNF) consisted of tumor-targeting aptamers and DNAzymes for silencing autophagy-related genes, with surface modification of low-dose photosensitizer (Ce6). It is found that the multifunctional DNF can specifically target tumor cells and generate reactive oxygen species (ROS) under light irradiation to trigger self-disassembly of DNF, enhancing the bioavailability of encoded DNAzymes, leading to amplified autophagy suppression. As a facile spatiotemporally programmable photogene therapy platform, the designed DNF is able to suppress tumor growth in vivo with a very low injection dose of Ce6 (18  µg kg−1, around 100 times lower than the generally applied dose), representing a promising strategy for cancer therapy with safely low-dose PDT.
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research

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CONCLUSION: In this research we proved that hybrids of benzocheromen and amino pyrimidine could be effective on growth inhibition of cancer cell lines and may be used as a drug candidate for cancer therapy in the future.PMID:34819010 | DOI:10.2174/1573406418666211124103504
Source: Medicinal Chemistry - Category: Chemistry Authors: Source Type: research
Near-infrared II (NIR-II) plasmonic phototheranostics, an “all-in-one” phototheranostic platform, is reported with plasmonic Au@Cu2 −xSe core-shell nanocrystals for photoacoustic-photothermal imaging-guided chemodynamic-photocatalytic-photothermal therapy all triggered by a single NIR-II laser. Tumor microenvironment-specific production of hydroxyl radials via Fenton-like reaction and photocatalytic reaction offers excellent therapeutic efficacy for cancer, achieving complete tumor eradication using a single NIR-II laser. AbstractTumor microenvironment (TME)-activatable phototheranostics is highly desirab...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research
This study thus contributes a novel concept for designing ultrasmall nanoparticle characteristics of preferential accumulation i n tumors, and also provides a strategy for creating high-performance carrier-free nanomedicine via highly ordered molecular stacking.
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research
Although breast cancer has been previously considered “cold” tumors, numerous studies are currently conducted to explore the great potentials of immunotherapies in improving breast cancer patient outcomes. In addition to the focus on stimulating adaptive immunity for antitumor responses, growing evidence showed the importance of triggering host innate immunity to eradicate established tumors and/or control tumor metastasis of breast cancer. In this review, we first briefly introduce the breast tumor immune microenvironment. We also discuss innate immune targets and pathways and mechanisms of their synergy with ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Autophagy and apoptosis regulate cell survival and death. Under normal conditions, autophagy is a homeostatic cytoprotective process. However, excessive autophagy is linked to cell death. In cancer, autophagy could rescue cells from drug induced death. Thus, the modulation of autophagy could be important in cancer therapy. We found that the acute phase protein alpha1-antitrypsin (AAT) rescues NSCLC lung cancer cell lines H1975 and H661 from cell death induced by staurosporine (STS), a cytotoxic drug simultaneously activating apoptosis and autophagy. Here, our aim was to analyze in more detail which steps in STS-induced cel...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Lung cancer Source Type: research
Conclusions: In conclusion the current data suggests that the miR-146a expression in PBMCs, and serum levels of TGF-β and IL-6 may suggests and predict the outcome of NSCLC patients.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Lung cancer Source Type: research
The early diagnosis of lung cancer remains a challenge and several trials are evaluating the feasibility and effectiveness of lung cancer screening. The SUMMIT study [NCT03934866] targets high risk individuals and offers low dose CT scanning. Abnormalities identified trigger referral to local hospitals for assessment.Our aim was to evaluate the potential impact, on cost and resources, of the introduction of lung cancer screening on our service. Data were collected retrospectively from electronic patient records for all referrals by the SUMMIT team. NHS Tariffs were used to evaluate referral costs.81 patient referrals were ...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Lung cancer Source Type: research
We would like to thank the authors Sekkate et  al. for their letter describing findings from a single-centre prospective study at Foch Hospital in France. Their aim was to evaluate anti-SARS-CoV-2 antibody titers in patients with solid tumours (excluding lung cancers) who had received two doses of the BNT162b2 vaccine and to assess the corre lation between these titers and lymphocyte count. Their study included 237 patients who had received two doses of the BNT162b2 vaccine. Blood samples for anti-spike antibodies and lymphocyte determinations were taken 3–4 months following vaccination.
Source: European Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Letter to the Editor Source Type: research
Eur J Pharm Sci. 2021 Nov 21:106081. doi: 10.1016/j.ejps.2021.106081. Online ahead of print.ABSTRACTPulmonary drug administration for the treatment of lung cancer is useful because the drug is directly delivered to the lung tissues with minimal invasiveness and higher efficiency compared to other conventional methods. However, it is critical to enhance drug accumulation in the lung cancer tissues to achieve sufficient therapeutic efficacy. The submicron-sized liposome (ssLip) preparation is one of the most promising approaches to enhance drug accumulation in the lungs; however, ssLips prepared for conventional inhalation d...
Source: European Journal of Pharmaceutical Sciences - Category: Drugs & Pharmacology Authors: Source Type: research
The capacity of T cells to recognize and mount an immune response against tumor antigens depends on the large diversity of the T-cell receptor (TCR) repertoire generated in the thymus during the process of T-cell development. However, this process is dramatically impaired by immunological insults, such as that caused by cytoreductive cancer therapies and infections, and by the physiological decline of thymic function with age. Defective thymic function and a skewed TCR repertoire can have significant clinical consequences. The presence of an adequate pool of T cells capable of recognizing specific tumor antigens is a prere...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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