Characterization of extracellular secondary metabolites in Oudemansiella canarii BRM-044600 displaying antifungal activity against the phytopathogen Sclerotinia sclerotiorum

The objective of this study was to screen for basidiomycete fungi capable of inhibitingS. sclerotiorum and investigate their bioactive metabolites responsible for antifungal activities. Two out of 17 tested basidiomycete isolates inhibited the mycelial growth ofS. sclerotiorum in pair culture experiments on agar plates, namelyOudemansiella canarii BRM-044600 andLaetisaria arvalis ATCC52088.O. canarii BRM-044600 liquid culture filtrate exhibited the greatest antifungal activity and was selected for further investigation. UHPLC-MS analysis suggests that six putative strobilurins, including strobilurin A and/or stereoisomers of this compound (m/z 259.1299, [M  + H]+) and three putative strobilurins with m/z 257.1184 ([M  + H]+) are likely responsible for the antifungal activity observed in the culture filtrate. For the first time, this work demonstrated the potential ofO. canarii for white mold biocontrol and strobilurin production.Graphic abstract
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research