A Novel Cascade Nanoreactor Integrating Two ‐Dimensional Pd‐Ru Nanozyme, Uricase and Red Blood Cell Membrane for Highly Efficient Hyperuricemia Treatment

A cascade nanoreactor, Pd-Ru/Uricase@RBC, is fabricated by integrating Pd-Ru with uricase and red blood cell (RBC) membrane for hyperuricemia therapy. By combining uricase activity with the catalase-like activity of Pd-Ru, the Pd-Ru/Uricase@RBC can effectively degrade uric acid to allantoin and H2O2, and subsequently catalyze the product H2O2 to O2, thereby realizing safe and efficient hyperuricemia treatment. AbstractNanozyme-based cascade reaction has emerged as an effective strategy for disease treatment because of its high efficiency and low side effects. Herein, a new and highly active two-dimensional Pd-Ru nanozyme is prepared and then integrated with uricase and red blood cell (RBC) membrane to fabricate a tandem nanoreactor, Pd-Ru/Uricase@RBC, for hyperuricemia treatment. The designed Pd-Ru/Uricase@RBC nanoreactor displayed not only good stability against extreme pH, temperature and proteolytic degradation, but also long circulation half-life and excellent safety. The nanoreactor can effectively degrade UA by uricase to allantoin and H2O2 and remove H2O2 by using Pd-Ru nanosheets (NSs) with the catalase (CAT)-like activity. More importantly, the finally produced O2 from H2O2 decomposition can in turn facilitate the catalytic oxidation of UA, as the degradation of UA is an O2 consumption process. By integrating the high-efficiency enzymatic activity, long circulation capability, and good biocompatibility, the designed Pd-Ru/Uricase@RBC can effectively and safely treat ...

Source: Small - October 20, 2021 Category: Nanotechnology Authors: Jiang Ming, Tianbao Zhu, Jingchao Li, Zichen Ye, Changrong Shi, Zhide Guo, Jingjuan Wang, Xiaolan Chen, Nanfen Zheng Tags: Research Article Source Type: research