Nanoarrays of Individual Liposomes and Bacterial Outer Membrane Vesicles by Liftoff Nanocontact Printing

Liposomes and extracellular vesicles are captured in nanoscale arrays via their surface biomarkers. Capture is enabled by creating antibody and toxin nanodot arrays with liftoff nanocontact printing. The nanodots are ≈425 nm in diameter, resulting in a maximum of one captured particle per nanodot. Individual particle resolution is maintained, and the number of particles per unit area is maximized. AbstractAnalytical characterization of small biological particles, such as extracellular vesicles (EVs), is complicated by their extreme heterogeneity in size, lipid, membrane protein, and cargo composition. Analysis of individual particles is essential for illuminating particle property distributions that are obscured by ensemble measurements. To enable high-throughput analysis of individual particles, liftoff nanocontact printing (LNCP) is used to define hexagonal antibody and toxin arrays that have a 425  nm dot size, on average, and 700 nm periodicity. The LNCP process is rapid, simple, and does not require access to specialized nanofabrication tools. These densely packed, highly ordered arrays are used to capture liposomes and bacterial outer membrane vesicles on the basis of their surface bioma rkers, with a maximum of one particle per array dot, resulting in densely packed arrays of particles. Despite the high particle density, the underlying antibody or toxin array ensured that neighboring individual particles are optically resolvable. Provided target particle biomarker...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research