Molecules, Vol. 26, Pages 6288: Pulsed Power Applications for Protein Conformational Change and the Permeabilization of Agricultural Products

Molecules, Vol. 26, Pages 6288: Pulsed Power Applications for Protein Conformational Change and the Permeabilization of Agricultural Products Molecules doi: 10.3390/molecules26206288 Authors: Koichi Takaki Katsuyuki Takahashi Alexis Guionet Takayuki Ohshima Pulsed electric fields (PEFs), which are generated by pulsed power technologies, are being tested for their applicability in food processing through protein conformational change and the poration of cell membranes. In this article, enzyme activity change and the permeabilization of agricultural products using pulsed power technologies are reviewed as novel, nonthermal food processes. Compact pulsed power systems have been developed with repetitive operation and moderate output power for application in food processing. Firstly, the compact pulsed power systems for the enzyme activity change and permeabilization are outlined. Exposure to electric fields affects hydrogen bonds in the secondary and tertiary structures of proteins; as a result, the protein conformation is induced to be changed. The conformational change induces an activity change in enzymes such as α-amylase and peroxidase. Secondly, the conformational change in proteins and the induced protein functional change are reviewed. The permeabilization of agricultural products is caused through the poration of cell membranes by applying PEFs produced by pulsed discharges. The permeabilization of cell membranes can be used for the extraction of nutrient...
Source: Molecules - Category: Chemistry Authors: Tags: Review Source Type: research