Invasive Streptococcus pyogenes disease in Spain: a microbiological and epidemiological study covering the period 2007 –2019

AbstractThe aim of this study is to present the first nationwide microbiological and epidemiological study of invasive group AStreptococcus (iGAS) disease in Spain. One thousand eight hundred ninety-three iGAS isolates were analyzed over 2007 –2019.emm typing was performed by sequencing the gene ’s variable 5’ end, exotoxin genes were identified by PCR, and antimicrobial susceptibility explored via theE test and disk diffusion. Five hundred twenty-three isolates were associated with sepsis, 292 with cellulitis, 232 with scarlet fever, 153 with pneumonia, 141 with streptococcal toxic shock syndrome, and 94 with necrotizing fasciitis. The most prevalentemm types wereemm1 (449/1893 isolates),emm89 (210/1893),emm3 (208/1893),emm4 (150/1893),emm12 (112/1893)emm6 (107/1893),emm87 (89/1893),emm28 (88/1893),emm75 (78/1893),emm77 (78/1893),emm11 (58/1893), andemm22 (35/1893).emm1,emm3,emm4, andemm6 were the predominant types affecting children (mostly respiratory infections), whileemm11,emm77, andemm89 prevailed in the elderly (mostly skin infections). Eachemm type was associated with one or more exotoxin gene (spe, sme, andssa) profiles.speA was detected in 660 isolates,speB in 1829,speC in 1014,speF in 1826,speG in 1651,speJ in 716,speH in 331,smeZ in 720, andssa in 512. Isolates withspeA were associated with the most severe infections. Penicillin susceptibility was universal. Two hundred twenty-four isolates were resistant to tetracycline, 169 to erythromycin, and 81 to clind...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research