Immunoglobulin Gene Clonality Analysis using Next-Generation Sequencing for Improved Minimal Residual Disease Detection with Significant Prognostic Value in Multiple Myeloma Patients

Next-generation sequencing (NGS) of rearranged immunoglobulin genes is an effective technology for identifying pathological clonal cells in multiple myeloma (MM) and tracking minimal residual disease (MRD). The clinical effect of implementing NGS in immunoglobulin gene clonality analysis was evaluated via a retrospective chart review. We enrolled a total of 312 patients diagnosed with MM. Immunoglobulin gene clonality was determined by fragment analysis using BIOMED-2 multiplex PCR assays (Invivoscribe Technologies) and by NGS using the LymphoTrack IGH FR1 Assay and LymphoTrack IGK Assay (Invivoscribe Technologies).

Source: Journal of Molecular Diagnostics - October 14, 2021 Category: Pathology Authors: Jihye Ha, Hyeonah Lee, Saeam Shin, Hyunsoo Cho, Haerim Chung, Ji Eun Jang, Soo-Jeong Kim, June-Won Cheong, Seung-Tae Lee, Jin Seok Kim, Jong Rak Choi Tags: Regular Article Source Type: research