Activity of the Giardia intestinalis Proteasome During Encystation and its Connection with the Expression of the Cyst Wall Protein 1 (CWP1)

This study assessed the proteasome activity in the trophozoite and during encystation. Strong activation of the proteasome was observed during the differentiation of trophozoites into cysts, reaching its maximum level 24 hours after the stimulus. We also found that the Giardia proteasome presents unusual characteristics related to higher eukaryotic proteasomes, making it an eventual therapeutic target. Here we tested the effects on the synthesis of a cyst wall protein by chemical inactivation of the proteasome and by overexpression or partial inhibition of the deubiquitinating protein RPN11 in transfected cells. Moreover, an analysis of the intracellular localization of RPN11 (an integral part of the proteasome regulatory particle) revealed major changes associated with the differentiation of trophozoites into cysts. This evidence further supports the important role of the proteasome in Giardia encystation.PMID:34627761 | DOI:10.1016/j.actatropica.2021.106183

Source: Acta Tropica - October 10, 2021 Category: Infectious Diseases Authors: Magda E Alvarado Jenny J Chaparro-Guti érrez Eliana P Calvo Luisa F Prada Mois és Wasserman Source Type: research