Genes, Vol. 12, Pages 1587: Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains

In this study, we applied the QTL-seq approach using an F2 population derived from the cross between IR62266 and Homcholasit (HSC) to rapidly identify the quantitative trait loci (QTL) that confers resistance to BLS caused by a Thai Xoc isolate, SP7-5. The results showed that a single genomic region at the beginning of chromosome 5 was highly associated with resistance to BLS. The gene xa5 was considered a potential candidate gene in this region since most associated single nucleotide polymorphisms (SNPs) were within this gene. A Kompetitive Allele-Specific PCR (KASP) marker was developed based on two consecutive functional SNPs in xa5 and validated in six F2 populations inoculated with another Thai Xoc isolate, 2NY2-2. The phenotypic variance explained by this marker (PVE) ranged from 59.04% to 70.84% in the six populations. These findings indicate that xa5 is a viable candidate gene for BLS resistance and may help in breeding programs for BLS resistance.

Source: Genes - October 9, 2021 Category: Genetics & Stem Cells Authors: Tripop Thianthavon Wanchana Aesomnuk Mutiara K. Pitaloka Wannapa Sattayachiti Yupin Sonsom Phakchana Nubankoh Srihunsa Malichan Kanamon Riangwong Vinitchan Ruanjaichon Theerayut Toojinda Samart Wanchana Siwaret Arikit Tags: Article Source Type: research