237 Impact of innate immunity on pemphigus vulgaris disease manifestation

Pemphigus vulgaris is a chronic autoimmune blistering disease characterized by antibody production against Desmogleins 3 (Dsg3) and 1 (Dsg1) causing acantholysis. Increased levels of innate cytokines detected in the blister fluid suggest a role of innate immune system activity. To elucidate the discrepancy between binding of pathogenic antibodies and a lack of blister formation, we focused on the role of innate cofactors supporting acantholysis. Here, we used a dispase-based keratinocyte-dissociation assay with a human immortalized keratinocyte cell line (HaCaT) and human primary keratinocytes.

Source: Journal of Investigative Dermatology - October 1, 2021 Category: Dermatology Authors: M.F. Schmidt, R.A. Eichkorn, MA Feoktistova, M. Hertl, J. Waschke, A.S. Yazdi Source Type: research