miRNA Profiling of Hungarian Regressive Wilms' Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

CONCLUSIONS We conclude that a downregulation of miR-184, miR-194-5p, and miR-203a expression is observed in both regressive and blastemal tumors, but larger-scale studies are needed to confirm whether the degree of their underexpression correlates with the number of blastemal elements in a sample. In most of our FFPE samples aged up to 9 years, RNA extraction provided miRNA with quantity and quality sufficient for qRT-PCR-based analysis, emphasizing the relevance of pathological archives as miRNA sources in future studies.PMID:34608109 | DOI:10.12659/MSM.932731

Source: Medical Science Monitor - October 5, 2021 Category: Research Authors: Gergely Bugly ó Zs ófia Magyar Éva Romicsné Görbe Rita B ánusz Monika Cs óka Tam ás Micsik M árta Mezei Jaxi Ayman Shawky Yani P éter Varga Zolt án Sápi B álint Nagy Source Type: research