Role of T cells during the cerebral infection with < i > Trypanosoma brucei < /i >
by Gabriela C. Olivera, Leonie Vetter, Chiara Tesoriero, Federico Del Gallo, Gustav Hedberg, Juan Basile, Martin E. Rottenberg
The infection byTrypanosoma brucei brucei (T.b.b.), a protozoan parasite, is characterized by an early-systemic stage followed by a late stage in which parasites invade the brain parenchyma in a T cell-dependent manner.
Here we found that early after infection effector-memory T cells were predominant among brain T cells, whereas, during the encephalitic stage T cells acquired a tissue resident memory phenotype (TRM) and expressed PD1. Both CD4 and CD8 T cells were independently redundant for the penetration ofT.b.b. and other leukocytes into the brain parenchyma. The role of lymphoid cells during theT.b.b. infection was studied by comparing T- and B-cell deficientrag1-/- and WT mice. Early after infection, parasites located in circumventricular organs, brain structures with increased vascular permeability, particularly in the median eminence (ME), paced closed to the sleep-wake regulatory arcuate nucleus of the hypothalamus (Arc). Whereas parasite levels in the ME were higher inrag1-/- than in WT mice, leukocytes were instead reduced.Rag1-/- infected mice showed increased levels ofmeca32 mRNA coding for a blood /hypothalamus endothelial molecule absent in the blood-brain-barrier (BBB). Both immune and metabolic transcripts were elevated in the ME/Arc of WT andrag1-/- mice early after infection, except forifng mRNA, which levels were only increased i...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Gabriela C. Olivera Source Type: research
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