Efficient detection of SARS-CoV-2 from exhaled breath.
SARS-CoV-2 is transmitted through airborne particles in exhaled breath, causing severe respiratory disease. SARS-CoV-2 is typically collected by nasopharyngeal swab and detected by PCR; however, patients can test positive for 3 months after infection. Without the ability to assay SARS-CoV-2 in human breath, it is not possible to learn the transmission risk from infected individuals. To detect virus in breath, the Bubbler - a breathalyzer that reverse transcribes RNA from airborne SARS-CoV-2 particles into a sample-specific barcoded cDNA was developed.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Chaorui Duan, Luke Buerer, Jing Wang, Samuel Kaplan, Gavin Sabalewski, Gregory D. Jay, Sean Monaghan, Andrea E. Arena, William G. Fairbrother Tags: Regular Article Source Type: research