Evaluation of the QuantiFERON SARS-CoV-2 Interferon- ɣ release assay in mRNA-1273 vaccinated health care workers

J Virol Methods. 2021 Sep 20:114295. doi: 10.1016/j.jviromet.2021.114295. Online ahead of print.ABSTRACTCurrent studies focus on cellular and humoral immunity induced by novel SARS-CoV-2 vaccines. Non-responders to vaccinations are not uncommonly encountered in clinical medicine (e.g. in the field of hepatitis B). Whereas vaccine-induced humoral immunity against SARS-CoV-2 is compromised by emerging Variants of Concern (VOCs), cellular immunity against SARS-CoV-2 is emerging as resilient against VOCs. Thus commercially available test kits for diagnostic laboratories designed to evaluate cellular immune responses to SARS-CoV-2 are urgently needed. Here we evaluated the novel QuantiFERON SARS-CoV-2 assay (Qiagen) measuring INF-ɣ release induced by two spike-derived peptide pools (Ag1 and Ag2) in a cohort of health care workers vaccinated with the mRNA-1273 vaccine and confirmed humoral response. Our study indicates the usefulness of this novel assay for routine laboratories to evaluate cellular immunity against SARS-CoV-2 in response to mRNA-1273 vaccination.PMID:34555429 | PMC:PMC8452154 | DOI:10.1016/j.jviromet.2021.114295
Source: Journal of Virological Methods - Category: Virology Authors: Source Type: research