Molecules, Vol. 26, Pages 5804: MicroRNA Delivery by Graphene-Based Complexes into Glioblastoma Cells

The objective of this study was to use graphene-based complexes to induce deregulation of miRNA level in GBM cancer cells and to regulate the selected gene expression involved in apoptosis. The complexes were characterised by Fourier transform infrared spectroscopy (FTIR), scanning transmission electron microscopy and zeta potential. The efficiency of miRNA delivery to the cancer cells was analysed by flow cytometry. The effect of the anticancer activity of graphene-based complexes functionalised by the miRNA sequence was analysed using 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxyanilide salt (XTT) assays at the gene expression level. The results partly explain the mechanisms of miRNA deregulation stress, which is affected by graphene-based complexes together with the forced transport of mimic miR-124, miR-137 and antisense miR-21, -221 and -222 as an anticancer supportive therapy.

Source: Molecules - September 25, 2021 Category: Chemistry Authors: Marta Kutwin Malwina Ewa Sosnowska Barbara Strojny-Cie ślak Slawomir Jaworski Maciej Trzaskowski Mateusz Wierzbicki Andre Chwalibog Ewa Sawosz Tags: Article Source Type: research